guide the discussion

Academic supervisors:
This checklist is intended to guide the discussion that you MUST have with your project
students when they are completing their risk assessment form(s).
Tick the boxes once you have covered all the relevant points.
Does the student understand why the risk assessment process
must be undertaken?
Is the student aware that no work with either chemicals or lab
equipment can be undertaken if it has not been considered on their
risk assessment?
Is the student aware that any changes to the method that this risk
assessment refers to will result in this risk assessment being
reviewed and amended as necessary?
Have the risks associated with all chemicals that are to be used in
this work been considered and discussed?
Have only the hazardous chemicals been included on this risk
assessment?
Have areas of work that require specialist training been identified
and training with the appropriate person arranged?
e.g. use of the
cryostat, use of floor standing centrifuges.
Is the student aware of the consequences of non-compliance and
the systems that are in place for reporting any concerns / noncompliances?

Faculty of Health and Wellbeing – Biosciences/BMRC
RESARCH/PROJECT RISK ASSESSMENT FORM

PLEASE ENSURE ALL SECTIONS ARE COMPLETED.
No work covered by this assessment can be undertaken until it is logged by a senior member of the Technical Staff and a
Risk Assessment code allocated.
It is the responsibility of the person supervising the research to ensure that risk assessments are carried out, remain valid
through the review process and that the control measures are identified and applied. All forms must be generated
electronically and not handwritten.

Risk Assessment code (to be assigned by technical staff): Valid from: Expiry:

 

Person supervising this research (academic supervisor)/Author of this risk assessment.
Name: Signature:
This procedure has been assessed and any control measures identified will be used by the person(s) carrying out this
work.

 

Person carrying out this work.
If multiple people are carrying out this work, then signatures can be added to the sheet at the end of this assessment.
Name:
I have read, understood and will comply with the assessment details.
(Signature):
Position of study/work (please click appropriate box).
Undergraduate. ☐☐ Post doctoral. ☐☐
Post graduate. MSc ☐☐
PhD ☐☐
Placement/studentship. ☐☐
Technical staff. ☐☐ Academic staff. ☐☐

 

Title of project (Overall project this Risk assessment relates to).
Detail of activity (Description of the experimental procedures). Each different procedure must have a separate Risk assessment.
mendments / Review
The person carrying out this work must ensure that this risk assessment remains valid. Reviews should be carried out
annually or sooner if circumstances change.
This is an Click to select to risk assessment code:
Date review conducted: Click here to enter a date. Review conducted by:
Sections amended:
If section 4 has been amended please state name of chemicals added:

The following points must be considered before completing the subsequent sections of this form
Substitution or elimination of any of the hazardous substances where possible (e.g. use of sybr safe instead of
Ethidium bromide)
Changing any of the experimental process to remove the operation resulting in risk to exposure.
Reducing the quantities of hazardous substances that are ordered, stored, used and produced as waste.
Security.
The laboratories on Level 8 Owen and Norfolk are kept locked when not in use. The corridors are under magna lock
control from 6pm – 8am each day.
The laboratories on level 7 Norfolk (BMRC) are always under the control of the magna lock in the corridor. The doors to
the labs are left unlocked. Staff adopt a challenge culture with regard to non-departmental persons trying to access these
areas.
1. Identification of those at risk
In most cases, all boxes will be ticked, particularly if work is being carried out in teaching laboratories.
Who else may be at risk? (Click all boxes that apply).

Students (UG, PG, PhD). Academic/Research staff Cleaners
Bioscience Technical staff SHU maintenance staff including IT staff. Visitors
ontrol measures (Click all that apply);
Verbally inform personnel of the nature of the work being carried out
Ask personnel to leave if necessary
Restrict access (In addition to that outlined above

Are there any hazardous substances that carry a risk to pregnant women?

Yes ☐☐ If Yes, list the hazardous substance here:
No ☐☐

2. Legislative control
DSEAR – Dangerous Substances and Explosive Atmosphere Regulations.
The laboratories and plant rooms (700N and 820 Owen) have been assessed under the DSEAR guidelines. This ensures the
safe storage and use of substances that are flammable or potentially explosive (e.g Acetylene, Hydrogen, Methane and
Propene cylinders and highly flammable substances.)
These cylinders must not be placed into laboratories for use.
Are there any Radionuclides being used?

Yes ☐☐ If yes, then the faculty Radiation Protection Supervisor must be contacted for further details as a separate Radiological
Risk Assessment must be undertaken.
No
☐☐

Does this protocol involve any genetic modification or use cells/material that has been genetically modified?

Yes No If yes, please enter the project registration number(s) that cover this work;
If the protocol does not have a registration number with regard to Genetic modification work, please see the Departmental Bio
safety officer. All GM work must be approved by the GM committee and fully risk assessed independently of this risk
assessment.

3. Information, instruction and training/supervision
Describe the information, instruction and training requirements for those working with the hazardous substances,
microorganisms, cells and equipment outlined in this assessment. If training is required, it must be complete prior to the
work commencing.

Nature of work requiring training Training to be given by (outline which member of staff will
provide training).
Additional information:

Based on the competency of the individuals undertaking this work, please outline what level of supervision is required for
the overall activity by clicking on the appropriate box. Consider all workers individually. Refer to the guidelines at the end
of this form for definitions.

Name of worker Direct supervision Indirect local
supervision
Remote
supervision
No supervision
required.

Last Revised: January 2019 4
4. Hazardous substances (Chemicals)
Please refer to the guidelines at the end of this form before completing this table. (Add additional rows as necessary to this
table).

Hazardous
Substance
Quantity
(Volume or
weight)
For the stock,
outline the
volume of the
original
container and
then the
Hazards
Outline the hazard and
precautionary phrases (H and P)
with both numbers and
description. Some precautionary
phrases will be used here, some
are for the emergency procedures
Chemical storage
group
Outline the storage
arrangements.
Refer to the
guidelines at the
back and the
chemical storage
posters in stores and
Does this substance
need to be used in
a
fume hood?
Please select from
Disposal
Please select
from drop
down menu
Emergency procedures.
Outline the local procedures for
accidental spillage or release and
personal injury (contact with skin, in
eyes, swallowed etc.)
It may be that the original container
has been spilt so consider the
maximum volume of spillage that

 

amount that
will actually
be used.
column. around the
laboratories.
drop down menu could occur.
As supplied.
(If liquid state
conc.)
Storage group: Click here to
select
Click here to
select
Spill:
Personal injury:
Working
solution:
(State conc. &
Solvent)
Storage group: Click here to
select
Click here to
select
Spill:
Personal injury:
As supplied.
(If liquid state
conc.)
Storage group: Click here to
select
Click here to
select
Spill:
Personal injury:
Working
solution:
(State conc. &
Solvent)
Storage group: Click here to
select
Click here to
select
Spill:
Personal injury:
As supplied.
(If liquid state
conc.)
Storage group: Click here to
select
Click here to
select
Spill:
Personal injury:
Working
solution:
(State conc. &
Solvent)
Storage group: Click here to
select
Click here to
select
Spill:
Personal injury:

4. Hazardous substances (Chemicals) continued

Hazardous Substance Quantity
(Volume or weight)
Hazards Storage Fume hood
Please select from drop
down menu
Disposal
Please select from drop
down menu
Emergency procedures.
As supplied.
(If liquid state conc.)
Storage group: Click here to select Click here to select Spill:
Personal injury:
Working solution:
(State conc. & Solvent)
Storage group: Click here to select Click here to select Spill:
Personal injury:
As supplied.
(If liquid state conc.)
Storage group: Click here to select Click here to select Spill:
Personal injury:
Working solution:
(State conc. & Solvent)
Storage group: Click here to select Click here to select Spill:

 

Personal injury:
As supplied.
(If liquid state conc.)
Storage group: Click here to select Click here to select Spill:
Personal injury:
Working solution:
(State conc. & Solvent)
Storage group: Click here to select Click here to select Spill:
Personal injury:

5. Microorganisms.
The Advisory Committee for Dangerous Pathogens (ACDP) categorises biological agents into four hazard groups. The laboratories
within the department of Biosciences and Chemistry are only authorised to support work up to and including Hazard group 2
organisms. For further information please see the guidance notes at the end of this document.

Microorganism ACDP
Hazard
Group
Source/origin
e.g. hospital
isolate,
commercial
strain
Type of media in
which the organism
will be cultured
(solid or liquid and
include
amount/volume)
Hazard
e.g. Biohazard,
antibiotic
resistance, hazards
to pregnant women.
Control Measures
These are in addition
to PPE and training. If
there are no additional
measures, please state
‘None’.
Disposal
Please select
from drop
down menu
Emergency procedures.
Outline the local procedures for
accidental spillage or release
.
Please select from drop down menu
Click here
to select
Click here to select
Click here
to select
Click here to select
Click here
to select
Click here to select

6. Cell lines, Tissues and body fluids – Ethical approval must be obtained for patient/participant samples of tissues, blood,
hair sweat etc. Please see section 9.

Cell line/ tissue
type/body fluid type
Source/origin
(hospital,
commercial
strain,
abattoir etc.)
Quantity
or
volume.
Hazard
e.g. biohazard, hazard to
pregnant women,
screened/unscreened
Control Measures
These are in addition
to PPE and training. If
there are no additional
measures, please state
‘None’.
Disposal
Please select
from drop
down menu
Emergency procedures
Outline the local procedures for
accidental spillage or release. Use P
phrases where necessary.
Click here to
select
Click here to select
Click here to
select
Click here to select
Click here to
select
Click here to select
Any additional information
(e.g. Hep B immunisation for
‘worker’ required).

Last Revised: January 2019 8
7. Personal Protective Equipment – This is in addition to lab coats which must be worn at all times.

Stage of the process that
PPE is required (outline
each step, or state if PPE
is only required in an
emergency)
Gloves.
Tick appropriate box
Eye/Face protection.
Tick appropriate box
Clothing ( In addition to lab
coats)
Tick appropriate box
Nitri
le
(EN
374-
2,
EN3
74-
3)
vinyl
(EN
455)
Cryo
(EN
511)
Heat
proo
f
(EN
388,
EN4
07)
Leat
her
(EN
388,
EN5
11,E
N42
0)
Gog
gles
(D16
6 –
349
B)
Safet
y
glass
es
(EN
166
1F)
Face
visor
(UV)
EN1
66 1-
F3,
EN1
66
389
BTS
Face
Viso
r
(N
2)
EN1
66 1-
F3,
EN1
66
389
BTS
Safet
y
shoe
s
(EN
345-
1)
Apro
ns
(No
BSE
N
num
ber)
High
vis
coat/
vest
(EN
471
class
2)
Ear
defe
nder
s
(EN
352)
Additional information:

8. Physical and Equipment hazards

Equipment name or physical
environment.
(e.g. scene of
crime set up, SDS
electrophoresis apparatus, use
of heat/hotplate)
Hazard
(Electrocution, pressure, weight, manual
handling, trip hazards, risk of burns or
boiling over)
Disposal
Please select from drop
down menu
Control measures
(refer to guidelines)
Sharps – Scalpels, needles,
cryostat blades, microtome
blades
Cut hazard, needle stick hazard. Sharps bin Do not re-sheath needles.
Do not attempt to put a used
scalpel back into the packet.
Dispose of needles and scalpels
directly into the sharps bin.
Store microtome and cryostat
blades in 50ml sealed falcon
tubes. Only give these blades to
users who have the items on their
risk assessment form.
Click here to select
Click here to select

9. Additional risks.
Does the work require materials to be obtained by non-standard procurement routes? For example, patient samples donated
from hospitals, purchase of animal tissue from the abattoir.

Yes ☐☐ If Yes, give details:
No ☐☐

Does the work involve out of hours or lone working? UG and MSc students are not allowed to work out of hours.

Yes ☐☐ If Yes, give details:
No ☐☐

Does the work require ethical approval (e.g. involves the use of human tissue, or human participants)

Yes ☐☐ If Yes, please insert the project registration number here:
No ☐☐

10. Risk Evaluation of the Overall Process. Select the most hazardous Chemical/Biohazard or activity to use as the basis for
determining the Risk Index (Be aware that certain combinations of hazardous substances may have synergistic/antagonistic
hazardous effects and should be considered when completing this section.)

LIKELIHOOD
SEV
ERIT
Y
1 2 3 4 5
Highly unlikely
or impossible that
incident will
occur
Not likely that
incident will
occur
Possible that
incident could
occur
Likely that
incident will
occur
Incident will
definitely occur
or occurs
frequently
1 Injury/illness not requiring
first aid
LOW LOW MEDIUM MEDIUM HIGH
2 Injury requiring medical
attention
LOW MEDIUM MEDIUM HIGH EXTREME
3 Injury/illness resulting in
less than 7 days absence
from work/study
MEDIUM MEDIUM HIGH EXTREME EXTREME
4 Injury/illness requiring
more than 7 days absence or
resulting in a fatality.
MEDIUM HIGH EXTREME EXTREME EXTREME
5 Injury/illness causing
multiple fatalities.
HIGH HIGH EXTREME EXTREME EXTREME

 

Low- Monitor the risks and the control measures.
Medium- Monitor the risks and maintain strict control measures
High – Review and introduce additional controls to lower the level of risk. Activity not to be carried out if worker will be
working alone (see the university’s lone working policy
https://staff.shu.ac.uk/HealthandSafety/documents/Lone%20working%20policyv-1.pdf)
Extreme – DO NOT PROCEED. Immediately review the activity and introduce further control measures to lower the risk.
Re-assess before proceeding.

 

What is the Risk Index for this procedure? Click here to select
If the Risk index is high then review the activity and control
measures.
Do not carry out this activity under conditions of lone
working
If the Risk Index is extreme, DO NOT PROCEED. Review
the activity and fully re-asses the procedure.

Risk Assessment number:
Declaration:
I have read, understood and will comply with the Risk Assessment
outlined above.

Name Staff/Student
number
Position/course of
study
Signature

Risk Assessment number:
Declaration:
I have read, understood and will comply with the Risk Assessment
outlined above.

Name Staff/student
number
Position/course of
study
Signature

Last Revised: January 2019 8
Faculty of Health and Wellbeing –
Biosciences
Risk Assessment Guidance Notes- Do not submit these 6
pages as they are just guidance for completion of RA form.
These notes provide further
information to aid the completion of the different sections of the Risk Assessment form.
Front page – Amendments / Review History –
It is the responsibility of the person carrying out the
experimental work to ensure that risk assessments remain valid. They must be reviewed annually or sooner if required. Please
select from the drop down list or fill in the box where applicable. Once a risk assessment has been amended or reviewed, a copy
must be submitted to the technical staff, to be logged.
All forms must be generated electronically, handwritten forms will not be accepted. It is advised that you save a copy of each risk
assessment form that you generate.
If any item is additional to a current risk assessment, then it must be added as an amendment and the whole form re-submitted via
the risk assessment box at the Help Desk (Level 7 Owen Building).
Section 2. Legislative control – DSEAR.

The Dangerous substances and Explosive Atmospheres Regulations 2002 (DSEAR) require employers to control the risks to
safety from fire and explosions. Dangerous substances are any hazardous substances used or present at work that could, if not
properly controlled, cause harm to people as a result of a fire or explosion. They can be found in nearly all workplaces and include
such things as solvents, paints, varnishes, flammable gases, such as liquid petroleum gas (LPG), dusts from machining and
sanding operations and dusts from foodstuffs.
DSEAR assessments have been carried out for all of the laboratories on level 7 and 8 and plant rooms 700N and 820 Owen.
If a substance or preparation is classified (ie. labelled as such) as explosive, oxidising, extremely flammable, highly flammable or
flammable then it is a
dangerous substance.
Examples include; Petrol, acetylene gas, diethyl ether and other flammable solvents, acids and ammonia solution.
Items must be stored correctly with consideration given to incompatibility of chemicals. For example,; flammable
substances such as petrol, ethanol and acetic acid should not be stored next to strong oxidisers such as nitric acid.
Flammable substances must be stored in a vented flammables cabinet.
Nitric acid must be stored in a vented Oxidisers cabinet.
Large volumes of dangerous substances (>2.5L) must be given to the technical team for storage in the outside chemical
store.
Further information about DSEAR can be found on the University’s Health and Safety website
https://portal.shu.ac.uk/departments/hrd/healthandsafety/lhcontrol/pages/dangerous-substances.aspx
Section 3. Information, instruction and training/supervision.
If training has been outlined as a control measure, then it is essential that formal training is carried out and recorded. Records may
take the form of local records kept with the person directing the research or they may be more formal with training being provided
by a University department such as Health and Safety Services.
The level of supervision required must be outlined.
Direct supervision – Worker is closely supervised at all times by an experienced person.
Indirect local supervision Worker is supervised by someone who is located within the same room/floor and who has
usually supplied written or verbal instructions to the worker.
Remote supervision – Worker is supervised at a distance with limited contact with the supervisor.
No supervision required – Worker is not required to be supervised at all due to either having a lot of experience or the work
having little or no risk.
Section 4. Hazardous substances (Chemicals).
Details of the chemical as supplied (including concentration where appropriate) and the working solutions are
required. Please outline the size of the original container of the substance as it was supplied by the
manufacturer, consideration needs to be given as to the course of action if this container was accidentally spilt
or released.
Identifying Hazards
Hazard information can be found on Material Safety Data Sheets (MSDS) which are available on manufacturers’ websites.

e.g. Sigma Aldrich – http://www.sigmaaldrich.com
Fisher Scientific – http://www.fisher.co.uk
VWR – http://uk.vwr.com/app/Home

Manufacturers’ catalogues (and websites) also provide hazard information in the form of Risk and Safety Phrases. Risk
and Safety Statements, also known as R/S statements, R/S numbers, R/S phrases, and R/S sentences, is a system of
hazard codes and phrases for labeling dangerous chemicals and compounds.
In 2015, the risk and safety statements were replaced by hazard statements and precautionary statements.
Classification, labelling and packaging of chemicals is now harmonised by the introduction of the UN globally
harmonised system of classification and labelling of chemicals
.
This link provides a tool for converting R and S phrases into the new H and P phrases –
http://ghs.dhigroup.com/PagesGHS/TranslationTool.aspx
For acetic acid the information from the sigma website is as follows:

Hazard statement(s)
H314 Causes severe skin burns and eye damage.
protection/ face protection.
H226 Flammable liquid and vapour.
water for several minutes. Remove
Precautionary statement(s)
P280 Wear protective gloves/ protective clothing/ eye
P305 + P351 + P338 IF IN EYES: Rinse cautiously with

contact lenses, if present and easy to do. Continue rinsing.
If a substance is being used for which there is no hazard information, for example legal highs, please seek advice from either your
academic supervisor or a senior member of the technical team.
Chemical Storage groups

Hazardous
Substance
Quantity
(Volume or
weight)
For the stock,
outline the
volume of the
original
container and
then the amount
that will actually
be used.
Hazards
Outline the hazard and
precautionary phrases
(H and P) with both
numbers and
description
Chemical storage group
Outline the storage
arrangements.
Refer to the guidelines at
the back and the chemical
storage posters in stores
and around the
laboratories.
Does this
substance need
to be used in a
fume hood?
Please select
from drop down
menu
Disposal
Please select
from drop
down menu
Emergency procedures.
Outline the local procedures for
accidental spillage or release.
It may be that the original container
has been spilt so consider the
maximum volume of spillage that
could occur.
As supplied.
(if liquid state conc.)
Acetic Acid
Concentrated
2.5L Flammable.H226 –
Flammable liquid 1
Corrosive – causes
severe burns – H314
Inhalation causes
damage to respiratory
tract.
Target organs – kidneys
Storage group: F
Lab No:714
Yes Sink – Fume
hood
Spillage
Evacuate area.
Contain spillage and if able, soak
up with inert absorbent material
(spill Kit) and dispose of according
to hazards.
Wash the spill site with soap and
water.
Personal injury
In case of contact with eyes or skin,
wash thoroughly with copious
amounts of water and see a first
aider before seeking medical
advice.
If swallowed do not induce
vomiting, rinse mouth with water
and see a first aider before seeking
medical advice.
Working solution:
(State conc. &
Solvent)
Acetic acid 0.2M
made up in water
16ml Not applicable due to
low concentration
In a vented cupboard at
room temperature
No Sink – Fume
hood
Spillage
Contain spillage, soak up with inert
absorbent material and dispose of
according to hazards.
Personal injury
In case of contact with eyes or skin,
wash thoroughly with copious
amounts of water and see a first
aider.

Section 4. Hazardous substances – Disposal routes.
The disposal information on MSDSs is usually aimed at industrial applications with large scale usage. The tick box list
consists of only those disposal routes that are available within the Department of Biosciences. Please tick the appropriate
box for each item using the table below as a guide.

Chemical waste streams Type of waste
Dispose of down a bench sink with
copious amounts of running water
Small quantities of non-hazardous aqueous solutions.
Hazardous substances which are not harmful to the environment.
Dispose of down a fume hood sink
with copious amounts of running
water.
Small quantities of hazardous aqueous solutions such as concentrated acids, but which
are
NOT harmful to the environment.
Flammable Waste Solvent Bottle
(ORANGE labelled bottles)
Flammable solvents
e.g. Ethanol, Acetone, Diethyl Ether, Methanol, Ethyl Acetate, Petroleum Ether,
Acetonitrile, Toluene
Halogenated Waste Solvent Bottle
(GREEN labelled bottles)
Halogenated solvents
e.g. Dichloromethane, Chloroform, Carbon tetrachloride
Specialist chemical disposal
-arranged by technical staff
Hazardous substances which are harmful to the environment.
Heavy metals and metallic compounds.
Hazardous substances immiscible in water or organic solvents,
Cyanides and azides
Carcinogens, mutagens and teratogens
Malodorous chemicals and lachrymators
Phenol solutions
Highly reactive chemicals – reacts violently with water, acids or solvents
Bromine
Yellow Incineration Bin (either in
fume hood or floor standing)
Solid waste for incineration
e.g. Acrylamide gels, gels stained with Ethidium Bromide, tubes and tips containing
chemical residues, agar plates, contaminated gloves, plastic ware contaminated with
cells or bacteria.
Sharps container. Hypodermic needles, scalpels, other blades. NOT TO BE USED FOR BROKEN
GLASS.

Section 4. Hazardous substances – Emergency procedures
Acceptable phrases – These can be copied & pasted into the risk assessment form.

Spillage
Do not let product enter drains.
Personal injury
In case of contact with eyes or skin, wash
thoroughly with copious amounts of water and
see a first aider.

 

Evacuate area. In case of contact with eyes or skin, wash
thoroughly with copious amounts of water, see a

first aider before seeking medical advice.

Ventilate area. If swallowed do not induce vomiting, rinse mouth
with water and see a first aider before seeking

medical advice.
Sweep up without raising dust and
place in a container for disposal.
If inhaled move person to fresh air and contact a
first aider.
Contain spillage, soak up with inert
absorbent material (spill kit) and
dispose of according to hazards.
If inhaled and not breathing, move to fresh air.
First aider to give artificial respiration or arrange
for an ambulance.
Wash the spill site with water.
Wash the spill site with soap and
water.
Tri reagent/Phenol spill or contact with skin = phenol is not water soluble. Seek
immediate first aid help.

Section 5. Microorganisms.
The Advisory Committee for Dangerous Pathogens (ACDP) categorises biological agents into four hazard groups. Please
see your supervisor or the technical staff if you are unsure which hazard group the microorganisms you’re working with
belong to. Further information can be found at
http://www.hse.gov.uk/pubns/misc208.pdf
http://www.hse.gov.uk/biosafety/biologagents.pdf
The laboratories within the department of biosciences can accommodate work carried out at containment level 2. Bioscience
staff and students can only work with microorganisms in Hazard groups 1 and 2.
Hazard
Group 1
An organism that is most unlikely to cause human disease.
Hazard
Group 2
An organism that may cause human disease and which might be a hazard to laboratory workers but is unlikely to spread to the
community. Laboratory exposure rarely produces infection and effective prophylaxis or effective treatments are usually available.
Hazard
Group 3
An organism that may cause severe human disease and present a serious hazard to laboratory workers. It may present a risk of
spread in the community but there is usually effective prophylaxis or treatment available.
Hazard
Group 4
An organism that causes severe human disease and is a serious hazard to laboratory workers. It may present a high risk of spread
in the community and there is usually no effective prophylaxis or treatment.

Biological waste streams Type of waste
Autoclave Liquid waste that may be resistant to bleach treatment
e.g. Liquid microbiological waste (including GM),
Treat with bleach then dispose of
down sink
Liquid waste that poses low risk of infection and is inactivated once treated with bleach e.g.
liquid cell culture waste (including GM).
Tissue disposal Freezer – BMRC
713
Human and animal blood, tissue and body fluids. This waste must be documented in the log
book by the freezer.
Incineration bin Solid items that have been contaminated with microbes e.g. Agar pates or plastic serological
pipettes

Section 6. Cell lines, tissues and body fluids.
As for working with microorganisms, the laboratories within Biosciences can accommodate work at containment level 2.
Work with cell lines, tissues and body fluids must be carried out in biological safety cabinets.
Consideration needs to be given as to whether cell lines carry any infectious agents, or could produce agents that may
stimulate allergic reactions in humans. These details must be outlined in the hazards column. Cell lines containing BBVs
(Blood borne viruses) cannot be used.
For commercially produce cell lines, please also refer to the information provided by the supplier.
Please refer to the HSE guidelines outlined above for further information.

Biological waste streams Type of waste
Autoclave Liquid waste that may be resistant to bleach treatment
e.g. Liquid microbiological waste (including GM)
Treat with bleach then dispose of
down sink
Liquid waste that poses low risk of infection and is inactivated once treated with bleach e.g.
liquid cell culture waste (including GM).
Tissue disposal Freezer – BMRC
713
Human and animal blood, tissue and body fluids. This waste must be documented in the log
book by the freezer.
Incineration bin Solid items that have been contaminated cell lines, tissues and body fluids e.g. plastic
serological pipettes, plastic tubes.

Section 7. Personal protective equipment.
This section is a tick box as the options presented are only those that are available within the bioscience laboratories. Please do not
state that breathing apparatus should be worn as this is not available and alternative control measures must be adopted. E.g. use in
a fume hood.
Section 8 – Physical and Equipment hazards.
Consider electrocution risks from equipment, risk of injury from broken glassware, microscope slides, scalpels and risks
associated with specific instrumentation
e.g. severe cuts from cryostat usage or burns from steamers and microwaved
items.
The hazards associated with sharps are included as standard on each risk assessment form.
Transportation of equipment needs to be considered as some equipment may need to be moved, manual handling training
may be required.
Control measures may include training for use of equipment ,manual handling training and risk assessment as well as
measures outlining use of PPE or removal of
the electricity source for example.
Training for some pieces of equipment must be formalised and documented i.e. the Cryostat and the floor standing
centrifuge.

Section 9 – Additional risks
Procurement using non-standard routes must be approved by the necessary budget holders. Staff and students should not use
personal money to purchase items and then seek reimbursement, as there are university procurement cards held by
members of staff that can be used to purchase goods from high street or online suppliers. Items must not be bought by
individuals directly from websites, all purchases must go through the technical staff. Individuals must not bring in items
‘from home’ for use in the laboratories. Items needed from the ‘high street’ must be purchased by the technical staff.
Out of hours working must be approved by the Director of Studies/Line manager and the head of the research centre in line
with the University Lone working policy or the local procedures/policy –
https://www.shu.ac.uk/research/ethics-integrityand-practice/guidance-and-legislation
Ethics approval must be sought if human tissue or participants are to be used in the experimental work. Work using patient
/participant samples such as tissues, blood, sweat, hair etc. needs to be approved by the ethics committee. Please refer to
https://www.shu.ac.uk/research/ethics-integrity-and-practice.
There are certain legal requirements that must be met. PLEASE INSERT THE PROJECT REGISTRATION NUMBER
IN SECTION 9.
Section 10 – Risk Evaluation
Use the chemical, biological or physical hazard that will give the highest the risk index.
Choose the level of likelihood and the level of severity and determine the final risk outcome.
Refer to the colour key and do not proceed if the activity falls within the Extreme area. Seek advice from a senior member
of technical staff.
Last Revised: January 2019 8